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My paragon bar is full but I didn't have the heart to betray Samara :P
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Neither did I, but save before hand, like all other loyalty powers it will become available to learn for all your characters forever once you get it. Though I made an extra save with that character with Morinth just in case. God, she really is a bitch, but at least you get to "romance" her. That goes about as well as you'd expect.
I really can't remember how I got past the colossus now, but I do remember that once you get close enough it stops recharging it's shields.
Something you might consider is using the retrain abilities research in the lab, and redistribute your talent points to those more appropriate for the mission, even just temporarily. Adrenalin burst, shield boost and disruptor ammo seem best.
It's weird to me that you already have Legion, since I save him for last because of the disaster that IFF brings down on the Normandy and I don't like losing anyone. But it's very funny when Tali meets him on Haestrom.